Initial-rate studies of a thermophilic glucokinase from Bacillus stearothermophilus
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چکیده
منابع مشابه
Purification and characterization of an acidic, thermophilic phytase from a newly isolated Geobacillus stearothermophilus strain DM12
Microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. In addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. Bacillus sp. DM12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...
متن کاملThe purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus.
Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation of extraneous material at pH 5.5, ion-exchange chromatography on DEAE-Sepharose, pseudo-affinity chromatography on Procion Brown H-3R-Sepharose 4B and gel filtration on Ultrogel AcA 34. The purified enzyme had a specific activity of about...
متن کاملThermostable, alkaline and detergent-tolerant lipase from a newly isolated thermophilic Bacillus stearothermophilus.
Lipases are the enzymes of choice for laundry detergent industries, owing to their triglyceride removing ability from the soiled fabric, which eventually reduces the usage of phosphate-based chemical cleansers in the detergent formulation. In this study, a novel thermo-alkaline lipase-producing strain identified as Bacillus stearothermophilus was isolated from the soil samples of olive oil mill...
متن کاملPurification and characterization of the thermophilic D-alanine carboxypeptidase from membranes of Bacillus stearothermophilus.
The D-alanine carboxypeptidase, the major penicillinbinding component in membranes of Bacillus stearothermophilus, was purified by covalent a5nity chromatography. The enzyme was solubilized in the nonionic detergent Triton X-100, bound to a column of a 6-aminopenicillanic acidSepharose derivative, and eluted upon treatment of the column with neutral hydroxylamine. The enzyme was thermophilic, p...
متن کاملCloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus.
A mannitol phosphotransferase system (PTS) was identified in Bacillus stearothermophilus by in vitro complementation with Escherichia coli EI, HPr, and IIA(Mtl). Degenerate primers based on regions of high amino acid similarity in the E. coli and Staphylococcus carnosus EII(Mt1) were used to develop a digoxigenin-labeled probe by PCR. Using this probe, we isolated three overlapping DNA fragment...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2480013